The 6800-18 Aquatic Measurement Room is a new measurement room of the LI-6800 Advanced Photosynthesis Fluorescence Measurement System, designed specifically for measuring steady-state carbon assimilation and chlorophyll fluorescence of algal suspensions and other samples. 6800-18 further expands the measurement range of LI-6800 samples.

The LI-6800 is a trusted instrument by top scientists worldwide for measuring carbon assimilation and chlorophyll fluorescence in terrestrial plant photosynthesis. LI-6800 is equipped with high-precision CO₂And H₂The O analyzer and automatic control system are widely used in exploring cutting-edge scientific aspects of photosynthetic physiology.

The 6800-18 Aquatic Measurement Laboratory has expanded its measurement capabilities to aquatic samples, allowing researchers to explore the scientific issues of photosynthesis in algal suspensions.

Working principle

The traditional method for measuring algal photosynthesis is based on the variation of dissolved oxygen concentration over time. Unlike it, LI-6800 is an open, steady-state gas exchange measurement system. During the measurement process, CO enters the chamber₂And O₂Maintain a constant concentration and other environmental factors.

The analyzer measures CO in the airflow entering and leaving the measurement room₂And H₂The concentration of O is used to calculate the photocatalytic rate of the liquid sample by calculating the concentration difference of the gas flow material.

After measuring the suspension sample as cell density, mass, or chlorophyll content, the photosynthetic carbon assimilation rate of the sample can be determined using µ mol CO₂cell^-1s^-1、 µmol CO₂mg^-1s^-1And µ mol CO₂µg^-1s^-1To express.

At the same time, 6800-18 also used pulse amplitude modulation (PAM) technology to measure the chlorophyll fluorescence of the sample. By integrating the measurement results from both aspects, obtain more comprehensive information on photosynthesis in the sample.

Main advantage

Automation and intelligence of measurement process: providing advanced settings and automated control of measurement environment and process; Convenient file management.

l BP user-defined automatic measurement program: Use Python language or built-in graphical programming interface to complete programming
l Provide pH sensor access: suitable for universal 12mm diameter pH electrodes
l Support gas intake ports to provide various required gas environments for samples
l Real time data graphic output, monitoring the entire measurement process
l Note information input function
l The data format consists of text and Excel files (including calculation formulas) for easy recalculation

application area

l Measure photosynthesis related parameters of microalgae and other samples, including net photosynthetic rate A, actual photochemical quantum efficiency Φ PSII, non photochemical quenching NPQ, receptor side closure degree 1-qL of photosystem II reaction center, etc
l Explore the physiological activities of any small aquatic organism such as algal suspensions, corals, mosses, lichens, etc

Technical Parameter

Sample chamber
Wetting material: 316 stainless steel, float glass, Viton fluororubber, PTFE， Silicone, Acetal
Chamber working volume: 0-20 mL, recommended sample volume of 15 mL

CO₂gas analyzer
Working principle: Non dispersive infrared analyzer (NDIR)
Accuracy: At 400 μ mol/mol, RMS≤0.1μmol/ mol@4s average signal
Measurement range: 0-3100 µ mol/mol

CO₂control
Range: 0-2000 µ mol/mol
Other gases can be connected through the user's gas inlet.

Fluorescence analyzer (6800-01A)
Red blue interaction light output: 0-3000 µ mol m^-2s^-1
Far red light output: 0-20 µ mol m^-2 s^-1
Saturated flash intensity: 0-16000 µ mol m^-2 s^-1
Red action light peak wavelength: 625 nm
Blue action light peak wavelength: 475 nm
Far red light peak wavelength: 735 nm

temperature
Working temperature: 0~50 ℃ (no direct sunlight, no icing)
Storage temperature: -20~60 ℃, keep the measuring room clean and dry
Temperature control: Self provided water bath, # 10-32 threaded connection to the measuring room

Operating liquid environment
Temperature: from freezing point to 50 ℃
Salinity: 0-35%

Auxiliary interface
PH meter (to be purchased separately): 12mm diameter O-ring seal, equipped with amplifier. PH probe based on passive glass electrode, BNC interface (nominal -59 mV/pH slope, user calibrated)
Septa: Silicone-PTFE septa

Origin and Manufacturer: LI-COR, USA